NANS7002 Nanophotonics: Fluorescence and Plasmon Controlled Fluores (JSS28) (2 cr)
During the past 20 years there has been a remarkable growth in the use of fluorescence in the biological sciences. Fluorescence spectroscopy and time-resolved fluorescence are considered to be primarily research tools in biochemistry and biophysics. This emphasis has changed, and the use of fluorescence has expanded. Fluorescence is now a dominant methodology used extensively in biotechnology, flow cytometry, medical diagnostics, DNA sequencing, forensics, and genetic analysis, to name a few. The lectures will deal with basics of steady-state and time-resolved fluorescence spectroscopy, instrumentation and data analysis. They will cover time-domain and frequency-domain measurements, anisotropy, quenching and Förster Resonance Energy Transfer (FRET). Next, the lectures cover advanced time-resolved fluorescence topics and data analysis. Applications of fluorescence in biophysics, sensing, plasmon-controlled fluorescence or material science are presented along with an introduction to fluorescence microscopy.
80% lectures attendance, 80% exercise completion.